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Figure 5 | Molecular Medicine

Figure 5

From: Effect of Recombinant α1-Antitrypsin Fc-Fused (AAT-Fc) Protein on the Inhibition of Inflammatory Cytokine Production and Streptozotocin-Induced Diabetes

Figure 5

Western blot for examining the inhibitory effect of AAT-Fc against elastase. IL-32γ (20 ng) was mixed with elastase (20 ng) in the presence or absence of plasma-derived AAT or AAT-Fc. The plasma-derived AAT blocked IL-32γ cleavage in a 1:5 molar ratio, but AAT-Fc failed to inhibit IL-32γ cleavage. The molar ratio of elastase and AAT in each reaction is indicated on the top. The elastase-cleaved IL-32γ product appeared as a band of approximately 20 kDa. Representative data from one of four independent experiments are shown.

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Molecular Medicine

ISSN: 1528-3658