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Figure 6 | Molecular Medicine

Figure 6

From: Effect of Recombinant α1-Antitrypsin Fc-Fused (AAT-Fc) Protein on the Inhibition of Inflammatory Cytokine Production and Streptozotocin-Induced Diabetes

Figure 6

Quantitative analysis of the proteinase inhibitory activity of AAT on elastase, with IL-32γ as the substrate. The IL-32γ concentrations of reactions containing IL-32γ (100 ng/mL) alone or reactions containing IL-32γ with elastase in the presence or absence of plasma-derived AAT (A) or AAT-Fc (B) were measured using ELISA, as described in Materials and Methods. The molar ratio of elastase and plasma-derived AAT in the reaction is indicated. In the absence of plasma-derived AAT, only 15% of IL-32γ remained at the end of the reaction, whereas plasma-derived AAT completely blocked IL-32γ cleavage (A). However, AAT-Fc failed to block IL-32γ cleavage by elastase (B). Representative data from one of five independent experiments are shown.

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Molecular Medicine

ISSN: 1528-3658