| 10 µmol/L Met-ENK | 10 µmol/L MEGY | 10 µmol/L β-END |
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24 hpf | 1.03 ± 0.11 | 0.78 ± 0.17 | 0.18 ± 0.01a |
48 hpf | 1.11 ± 0.20 | 0.47 ± 0.07a | 0.98 ± 0.09 |
- Relative dre-oprm1 expression fold-changes in embryos that were exposed to a dose of 10 µmol/L opioid peptides (Met-ENK, MEGY and β-END) for 24 and 48 hpf are shown. Data are normalized to dre-eef1a1l1. A total of 50 embryos were used for each treatment and for each time point; the experiment was performed in triplicate and repeated three times.
- ap < 0.05 (unpaired Student t test, n = 3).