Figure 2

BHV1gG-Ig inhibits CXCL13 and CXCL1 but not CCL2 function in vitro. 0.2 µg/mL of recombinant CXCL13 together with 2 µg/mL (A), 8 µg/mL (B) or 16 µg/mL (C) of BHV1gG-Ig (black) was added to peritoneal cells from NZW mice. For control experiments, BAFF-R-Ig (dark gray) at the same concentration or control SCID serum (light gray) was used. Calcium flux was measured using flow cytometry. Data is representative of four experiments. (D) Mouse peritoneal cells (1 × 10⁶) were placed in each of the upper chambers of a transwell. Medium containing 1 µg/mL recombinant mouse CXCL13 together with 40 µg/mL of BHV1gG-Ig (n = 4), 40 µg/mL of BAFF-R-Ig (n = 4) or medium alone (n = 1) was placed in the lower chambers. Data represent the percentage of B220⁺ cells in the lower chamber divided by the total input of B220⁺ cells after 3 h of incubation at 37°C. (E) Sorted mouse BM neutrophils (2 × 10⁵) were placed in each of the upper chambers of a transwell. Medium containing 100 ng/mL of recombinant mouse CXCL1 together with 0, 0.25, 1 or 4 µg/mL (5.7 mol/L ratio) of BHV1gG-Ig or 4 µg/mL of BAFF-R-Ig was placed in the lower chambers. Data represent the percentage of Gr1^highCD11b^high cells in the lower chamber divided by the total input of Gr1^highCD11b^high cells after 1 h of incubation at 37°C. (F) Mouse peritoneal cells (2 × 10⁵) were placed in each of the upper chambers of a transwell. Medium containing 10 ng/mL of recombinant mouse CCL2 together with 0, 25, 100 or 400 ng/mL (5.7 mol/L ratio) of BHV1gG-Ig or 400 ng/mL of BAFF-R-Ig was placed in the lower chambers. Data represent the mean numbers of cells that attached to the membrane after 4 h of incubation at 37°C. *P < 0.05. Data are representative of three to four experiments.