Figure 1
From: Replacement of the C6ORF66 Assembly Factor (NDUFAF4) Restores Complex I Activity in Patient Cells
Schematic representation of TAT-ORF and its purification. (A) Schematic representation of TAT-ORF fusion protein. SDS-PAGE (B) and Western blots using anti-His antibodies (C) or anti-C6ORF66 antibodies (D) to analyze the purification steps of TAT-ORF by affinity chromatography as described in Materials and Methods are shown. Arrows indicate the TAT-ORF fusion protein. FT1, flow through 1; FT2, flow through 2; His, 6xhis tag; M., marker; MTS, mitochondrial targeting sequence; Sol., soluble fraction; TAT, Tat peptide.