Figure 5

Complex I activity in TAT-ORF-treated cells: calibration studies. (A, B) Complex I activity in F528 cells treated with TAT-ORF fusion protein (0.022 µg/µL, final concentration) as a function of the amount of mitochondria (A): 1.67 µg protein (1), 3.35 µg (2), 5 µg (3) of isolated mitochondria and incubation time (B). (C,D) Complex I activity in F528 cells treated with TAT-ORF fusion protein as a function of the start concentration of the TAT-ORF fusion protein (C) and final concentration in the assay (D). Incubation was performed for 24 h. Complex I activity was measured as described in Material and Methods. Activity values are expressed as nmol/min/mg protein and presented as average of triplicates ± SD.