Figure 4

Hepatic FoxO1 is distributed in both the cytosol and nucleus in the ebb phase of burn injury. (A) Distribution of hepatic FoxO1 determined by subcellular fractionation. Nuclear/cytosolic ratio of FoxO1 as determined by densitometry >1 indicates more nuclear distribution of FoxO1, whereas <1 indicates a more cytosolic distribution. Lamin B1 was used as a nuclear specific marker and LDH was used as a cytosolic-specific marker. Experiments were repeated in five independent cohorts. *Nonspecific band. (B) Real-time RT PCR gene expression of the hepatic FoxO1 coactivator PGC1-α. Data are shown as mean ± SEM.