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Figure 3 | Molecular Medicine

Figure 3

From: Molecular Expression and Characterization of Erythroid-Specific 5-Aminolevulinate Synthase Gain-of-Function Mutations Causing X-Linked Protoporphyria

Figure 3

SDS-PAGE analysis of succinyl CoA synthetase (SCS) binding to wild-type and Q548X ALAS2. Protein samples from before and after amylose affinity chromatography were visualized by SDS-PAGE. Molecular mass standards were run in lanes 1 and 9. FPLC-purified wild-type MBP-ALAS2, SCS and MBP-Q548X proteins were run in lanes 2, 5 and 8, respectively. Lanes 3 and 7 contained samples of the flow-through of unbound SCS applied to amylose columns containing bound WT-ALAS2 and Q548X MBP fusion proteins, respectively. Lanes 4 and 6 contained samples of the elution of these respective columns with maltose.

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Molecular Medicine

ISSN: 1528-3658