ALAS2 enzyme | Step | Activity (units) | SA (units/mg) | Purification (-fold) | Yield |
---|---|---|---|---|---|
Wild type | |||||
Crude extract | 775,000 | 1,520 | 1 | 100 | |
Amylose affinity | 374,000 | 19,200 | 13 | 48 | |
Gel filtration | 154,000 | 81,700 | 54 | 20 | |
ΔAT | |||||
Crude extract | 3,660,000 | 5,040 | 1 | 100 | |
Amylose affinity | 1,890,000 | 39,000 | 8 | 52 | |
Gel filtration | 767,000 | 148,000 | 29 | 21 | |
ΔAGTG | |||||
Crude extract | 3,660,000 | 6,130 | 1 | 100 | |
Amylose affinity | 2,590,000 | 51,500 | 8 | 71 | |
Gel filtration | 896,000 | 252,000 | 41 | 24 | |
ΔG | |||||
Crude extract | 1,110,000 | 1,760 | 1 | 100 | |
Amylose affinity | 769,000 | 15,300 | 9 | 69 | |
Gel filtration | 620,000 | 77,500 | 44 | 56 | |
p.F557X | |||||
Crude extract | 1,840,000 | 2,290 | 1 | 100 | |
Amylose affinity | 970,000 | 29,500 | 13 | 53 | |
Gel filtration | 491,000 | 62,900 | 27 | 27 | |
p.Q548X | |||||
Crude extract | 4,240,000 | 7,360 | 1 | 100 | |
Amylose affinity | 2,560,000 | 65,300 | 9 | 60 | |
Gel filtration | 2,040,000 | 388,000 | 53 | 48 | |
p.Q581X | |||||
Crude extract | 567,000 | 610 | 1 | 100 | |
Amylose affinity | 275,000 | 7,820 | 13 | 48 | |
Gel filtration | 210,000 | 69,300 | 114 | 37 |