Figure 3

miR-15a/MAPK8 and miR-101/FOS are negatively correlated at mRNA but not at protein level. MAPK8 (JNK) is the target with the highest prediction score for miR-15a. (A, C) Correlation and linear regression between miR-15a/MAPK8 and miR-101/FOS mRNA levels, respectively. ΔCt values were used and the correlation is statistically significant (p < 0.01). The slope in both cases is negative in both cases, which means that when miRNA expression is upregulated, mRNA expression is downregulated. (B, D) Protein levels of MAPK8 and FOS in representative M/U-CLL and subset 1 and 4 cases, respectively. MAPK8 is overexpressed in U-CLL. (E) miR-101 forced overexpression in one subset 1 case by using miR-101 mimic induced FOS protein downregulation compared with untransfected cells or cells transfected with negative control. (F) Transfection of subset 4 cells with miR-101 inhibitor induced FOS downregulation, yet to a lesser extent compared with miR-101-forced overexpression.