Figure 5

CD38 expression on fresh myelomas (A) and human myeloma cell lines (B) after 24 h exposure to ATRA. CD38 expression on fresh myelomas (C) and human myeloma cell lines (D) after 24 h exposure to tamibarotene. Cells were cultured at 37°C with retinoid for 24 h. Cells were then incubated at 4°C for 20 min with the appropriate conjugated antibodies panel. Flow cytometric analyses were performed using a FACScan flow cytometer (Becton Dickinson, San Jose, CA, USA), using a WinMDI 2.9 software.