Specific Nrf2 inducer activates ALR expression. (A) mRNA expression of NQO1 and ALR was analyzed after stimulation with tBHQ (200 µmol/L, 24 h) in HepG2 and PHH by qRT-PCR. Untreated cells served as control and 18S ribosomal RNA expression was determined for normalization (two independent experiments in triplicates, mean ± SD). (B) ALR protein expression in cellular lysates of HepG2 cells stimulated without or with tBHQ (200 µmol/L, 24 h) was analyzed by Western blotting followed by densitometry. *P < 0.05 differs from corresponding control.