Figure 3

Reduced expression of ARE-regulated genes in Nrf2^−/− mice after partial hepatectomy. A two-thirds partial hepatectomy using Nrf2 WT or Nrf2^−/− mice was performed inducing liver regeneration. Liver tissue samples were taken at indicated time points after surgery. (A–C) mRNA expression of NQO1 (A) and GST (B), and ALR (C) was determined by quantitative RT-PCR analysis. 18S ribosomal RNA expression was measured for normalization (four independent experiments, mean ± SEM). (D) Western blot analysis of liver tissue homogenates from Nrf2 WT and Nrf2^−/− mice after two-thirds partial hepatectomy was performed followed by densitometric analysis of 21- and 23-kDa ALR band at the indicated time points (one of four mice are presented). *P < 0,05 Nrf2 WT differs from Nrf2^{−/− #}P< 0.05 differs from 0 h.