Figure 3

Diazepam tolerance and withdrawal in Baiap3 KO and WT mice. (A) Experimental design scheme. (B) Male diazepam-treated Baiap3 KO mice showed significantly faster improvement of performance on the rotarod, consistent with a more rapid development of tolerance to diazepam. (C) Rotarod performance of female mice was comparable between WT and KO. (D, E) Diazepam-naive Baiap3 KO mice display a higher PTZ-induced seizure propensity compared with WT (significant in females, strong tendency in males). (F, G) Flumazenil-induced diazepam withdrawal does not further increase PTZ-induced seizure propensity in Baiap3 KO mice. Seizure propensity of female mice became comparable between genotypes, pointing to a ceiling effect. Numbers tested: males, WT = 25, KO = 25; females, WT = 21, KO = 23, except for (D) and (E), where males, WT = 7, KO = 7; females, WT = 8; KO = 10. Mann-Whitney U test (D–G) and two-way repeated-measures ANOVA (B, C), including Bonferroni, testing applied. Means ± SEM are presented.