Figure 4

Increased basal network activity and lack of homeostatic adaptation to diazepam treatment in Baiap3 KO hypothalamus slices. (A) Sample traces of mIPSC recordings from WT and KO hypothalamus slices that were cultured in the presence of diazepam or under vehicle control conditions with DMSO. Under diazepam withdrawal conditions, Baiap3 WT mIPSC amplitudes were significantly smaller than in KO slices (B), and WT mIPSC rise times were longer than in KO slices (C). (D) Baiap3 WT slices showed an increase in AP frequency in response to diazepam withdrawal when compared with DMSO-treated WT slices, whereas no such increase was apparent for Baiap3 KO slices, which already showed an increased AP frequency under DMSO control conditions when compared with WT slices. (E) The resting membrane potential was not affected by experimental condition or Baiap3 genotype. IPSC amplitudes (F) and IPSC frequencies (G) were increased in Baiap3 WT slices under diazepam withdrawal compared with DMSO-treated WT slices. Mann-Whitney U test was used for AP and IPSC frequencies; Student t test was used for all other parameters. Means ± SEM are presented.