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Figure 4 | Molecular Medicine

Figure 4

From: Activated Protein C Inhibits Proliferation and Tumor Necrosis Factor α-Stimulated Activation of p38, c-Jun NH2-Terminal Kinase (JNK) and Akt in Rheumatoid Synovial Fibroblasts

Figure 4

APC downregulates TNFα-stimulated phosphorylation (P) of MAPKs p38, JNK and Akt in RSFs. RSFs were treated without (control (Cont.)) or with TNFα (2 ng/mL) in the presence or absence of APC (10 µg/mL). P-p38, p38 (A), P-JNK (B), P-Akt, Akt (C) and P-ERK1/2, ERK-1/2 (D) were detected by Western blotting. p38 or β-actin were used as a loading control. The band intensity of the proteins was normalized with p38 or β-actin, and TNFα was defined as 1. Values are shown as mean ± SD; n = 3, except (D), where the middle bar represent n = 4 and last bar represent n = 2; *p < 0.05 versus control; #p < 0.05 versus TNFα-treated RSF, with the use of a paired t test. APC pre, APC pretreatment.

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