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Figure 1 | Molecular Medicine

Figure 1

From: Enhanced Inducible Costimulator Ligand (ICOS-L) Expression on Dendritic Cells in Interleukin-10 Deficiency and Its Impact on T-Cell Subsets in Respiratory Tract Infection

Figure 1

ICOS-L expression on DCs is significantly higher in IL-10 KO than WT mice after Cm infection. DCs from IL-10 KO or WT mice with or without infection were isolated from spleens by using CD11c microbeads and LS MACS columns as described in Materials and Methods. DCs were stained with anti-CD11c-FITC and anti-ICOS-L-PE mAbs for 20 min on ice and analyzed by flow cytometry. (A) Representative histogram of ICOS-L expression on DCs. Solid line: DCs from IL-10 KO mice (KO); dashed line: DCs from WT mice (WT); shaded histogram: isotype control. (B) MFI of ICOS-L on DCs of naive and infected WT and IL-10 mice is shown as mean ± standard deviation (SD) (n = 4). **p < 0.01. One representative experiment of three independent experiments is shown.

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Molecular Medicine

ISSN: 1528-3658