Figure 2

IL-10 KO mice show less Tregs after Cm infection. IL-10 KO and WT mice were infected with Cm (1 × 10³ IFUs) as described in Material and Methods. At d 7 after infection, single cell suspensions of the spleen and lung were stained for expression of CD3e, CD4 and Foxp3. (A) Representative dot plots of Foxp3⁺CD4⁺ cells as a percentage of total T cells are shown. (B) Summary of the frequency of CD4⁺Foxp3⁺ T cells in spleen and lung. (C, D) Foxp3 mRNA in the lung tissues was analyzed by RT-PCR in IL-10 KO and WT mice. Data are shown as mean ± SD (n = 4) and are representative of three independent experiments with similar results. *p < 0.05, **p < 0.01.