Figure 7
Reduced Th17 but increased Th1 responses in ICOS KO mice compared with WT mice after Cm lung infection. ICOS KO or WT mice were infected with Cm (1 × 103 IFUs) intranasally, and single cell suspensions of spleen and lung cells were analyzed at d 7 after infection. IL-17 (A, B) or IFNγ-expressing CD4 T cells (C, D) were detected by intracellular cytokine staining. Representative flow cytometric plots (A, C) and summary of the results (B, D) are shown. *p < 0.05; **p < 0.01.