Figure 2

Temporal intraclonal dynamics of CLL subset 4. The level of ID observed over time could be described as increasing, decreasing or complex (that is, a mutation appears or disappears and then reemerges at a subsequent time point). (A) Graphs illustrate the patterns of clonal branching over time in both the IGHV4-34 and IGKV2-30 genes of subset 4 CLL cases. The relative size of each subclone is plotted against all time points analyzed, and within each case, a cluster of identical subcloned sequences or a unique subcloned sequence is represented by a different colored segment. TP, time point. (B) Schematic representation of the dynamic patterns of ID observed within the IGHV4-34 gene of case P1422. Although major and minor clones were observed initially within P1422, a dramatic shift in subclonal composition occurs at later time points, and the minor clone becomes undetectable. The majority of subcloned sequences at later time points retain the mutational pattern of the predominant clone, while individual clones that acquire additional mutations are indicated by the black circles. Additional graphs and sequence information are available in Supplementary Figures S1-S4 and Supplementary Tables S6–S8.