Skip to main content
Figure 1 | Molecular Medicine

Figure 1

From: Midkine Is Expressed and Differentially Processed during Chronic Obstructive Pulmonary Disease Exacerbations and Ventilator-Associated Pneumonia Associated with Staphylococcus aureus Infection

Figure 1

MK as detected by immunofluorescence in lung tissue from COPD. The immunoreactivity of MK expression, expressed as positive pixels per mm2, in the small airway wall (A), small airway epithelium (B) and alveolar parenchyma (C). Data expressed as scatter dot plots and line indicate the median value. Asterisks show statistical difference when compared with controls: *p < 0.05, **p < 0.01. Immunohistochemical staining of MK in small airways (D), small airway epithelium at higher magnification (E) and alveolar parenchyma (F). Triple fluorescent immunohistochemical staining of nuclei (DAPI, blue) and MK (Alexa Fluor 555, red) together with MPO of neutrophils show weak colocalization (G) and surfactant protein A (SP-A) expressed by type 2 pneumocytes of the alveoli and is shown in green (H and I: Alexa Fluor 488). In (I), alveolar macrophages containing SP-A (that is, likely to have been phagocytosed) also show presence of MK. Scale bars: D, F = 200 µm; E, H, I = 50 µm; and G = 100 µm.

Back to article page

Molecular Medicine

ISSN: 1528-3658