Figure 2

CBX effectively abrogated LPS-induced HMGB1 release in macrophage cultures. Murine macrophagelike RAW 264.7 cells (panel A) or primary peritoneal macrophages (panel B) were stimulated with LPS in the absence or presence of CBX at indicated concentrations. At 16 h after stimulation, the levels of HMGB1, TNF and nitric oxide in the culture medium were respectively determined by Western blotting, ELISA and Griess reactions, and expressed in arbitrary units (AU). *P < 0.05 versus negative control (− LPS); ^#P < 0.05 versus positive control (+ LPS alone).