Figure 5

CBX and P2X₇R antagonists inhibited LPS-induced dye uptake and HMGB1 release. A), CBX attenuated LPS-induced elevation of dye uptake. Murine macrophage cultures were stimulated with LPS for 16 h, and subsequently incubated with the LY dye for 15 min. The number of LY dye-positive cells were counted and expressed as a percentage of total number of cells in multiple fields. *P < 0.05 versus negative control; ^#P < 0.05 versus positive control (+ LPS alone). B, C), P2X₇R antagonists attenuated LPS-i nduced elevation of dye uptake and HMGB1 release. Macrophage cultures were stimulated with LPS (0.5 µg/mL) in the absence or presence of several P2X₇R antagonists, KN62 (0.5 µmol/L), BBG (0.5 µmol/L), and oATP (50.0 µmol/L). At 16 h after LPS stimulation, the P2X₇R activities were determined by the LY dye uptake assays, and the levels of HMGB1 in the culture medium were determined by Western blotting analysis. *P < 0.05 versus negative control (− LPS); ^#P < 0.05 versus positive control (+ LPS alone).