Figure 6

CBX attenuated LPS-induced PKR upregulation and phosphorylation in macrophage cultures. Primary peritoneal macrophages were stimulated with crude LPS in the absence or presence of CBX (panel A) or P2X₇R antagonists (oATP, panel B) for 16 h, and cellular levels of total or phosphorylated PKR were determined by Western blotting analysis with reference to a housekeeping protein, β-actin. *P< 0.05 versus negative control (− LPS and — CBX). #P < 0.05 versus positive controls (+ LPS alone).