Figure 1

Effects of metformin or AMPK inhibitor compound C on neutrophil Chemotaxis. (A, B, C) Representative images (A) and quantitative data (B, C) show neutrophil migration after dose- and time-dependent stimulation with W-peptide. In (B), transmigration assay was performed by inclusion of W-peptide (0),12.5, 25 or 50 nmol/L) for 60 min, whereas, in (C), migration was determined after inclusion of 50 nmol/L W-peptide for the indicated time period. Means ± SD (n = 3), ***P < 0.001, compared with untreated neutrophils. (D) Representative Western Blots and quantitative data show the amount of phosphorylated and total AMPK in neutrophils treated with W-peptide (50 nmol/L) for 0, 20, 40 or 60 min. Average (mean ± SEM) of optical bend density was obtained from three independent experiments (*P < 0.05, compared with untreated). (E, F) HL-60 cells were treated with IL-8 for 60 min followed by chemotaxis assay (E) and Western Blot analysis (F) for phospho and total AMPK. Means ± SD (n = 3), *P< 0.05, **P< 0.01, ***P< 0.001, compared with untreated cells.