Figure 6
From: Activation of AMPK Enhances Neutrophil Chemotaxis and Bacterial Killing
AMPK activation induces neutrophil cytoskeletal rearrangement and leading edge formation. Neutrophils were pretreated with AICAR (0 or 250 µmol/L) for 90 min or metformin (0 or 500 µmol/L) for 2.5 h followed by inclusion of LPS (0 or 1 µg/mL) for additional 90 min. (A,B) Representative images show actin (green) and nuclei (blue) staining (A), whereas (B) shows magnified region of interest. (C) Average of actin fluorescence is shown. Mean ± SD actin fluorescence intensity (n < 3 ∼ 5), *P < 0.05.