Figure 4

Correlation between CD27 expression and SHM in MZ B cells. (A) Splenic frozen sections are stained for the indicated markers and analyzed by confocal microscopy (20x). A higher magnification (30×) of the MZ is shown in the inset. (B) sIgD^lowCD38⁻ B cells were gated to separate sIgM^highCD27⁺ and sIgM^highCD27⁻ MZ B cells. The IGHV genes amplified from the two subsets were investigated for the presence of SHM. (C) Sorted sIgM^highCD27⁺ and sIgM^hgihCD27⁻ B cells were studied for the expression of the indicated markers (D). Staining of MZ B cells for anti-CD27 and -CD43.