Figure 5

Clonal diversification in MZ. (A) VH CDR3 logos of two different clonal families isolated from MZ B cells of spleen 1 are reported. The IGHD gene has been modified by SHM leading to a number of amino acid changes while conserved amino acids are found at N1 and N2 positions. The height of symbols within the stack indicates the relative frequency of each amino acid at that position. Amino acid position is according to IMGT numbering for the V domain. Logos were created by using the WebLogo tool (https://doi.org/weblogo.berkeley.edu). (B) Stereotyped sequences in spleen 1 and 4. They are identified as stereotyped based on the VH CDR3 similarities and utilization of the same V(D)J gene rearrangement. Amino acids in the boxes indicate spleen-specific SHM; arrows highlight the presence of common SHM on the IGHV gene when aligned with the closest germ line gene (IGHV1-69). VH CDR3 logos for each spleen are shown.