Figure 1
From: Immunomodulatory and Antibacterial Effects of Cystatin 9 against Francisella tularensis
rCST9 modulates MDM responses Ft S4. (A) MDM (5 × 105 in 200 µL media/sample) were treated concurrently with 50 pg of rCST9 then immediately infected with S4 (MOI of 40:1). At 24 h after infection and/or treatment, bacterial killing was significantly higher in rCST9-treated MDM, as compared with S4-infected MDM alone (P < 0.05). (B) rCST9 significantly decreased leukocyte migration in the presence of VEGF compared with VEGF alone (P < 0.05), as shown in a modified Boyden chamber assay. (C) Cells were stained to visualize and quantify migrated cells in the modified Boyden chamber assay. Data are presented as mean ± SEM. Asterisks signify significant differences of P < 0.05.