Peptides That Bind Specifically to an Antibody from a Chronic Lymphocytic Leukemia Clone Expressing Unmutated Immunoglobulin Variable Region Genes

Table 2 Isolated phage clones from selection with U-CLL mAb068.

Clone(s)	(Frequency)^a	Sequence^b	(A₄₅₀ (mAb068))/(A₄₅₀ (HSA))^c
B12, D7, E4, F9	(4)	NMSIATVP	28
C2, F3, F7, H11	(4)	ASCYIDDGIRYCTG	28
A6, B1, D2, E12	(4)	VSCNPFSWLPRCYA	19
A5, D4, D5	(3)	DYCNLSVSWCYP	26
F1, F5	(2)	STCWLMFSMCRV	24
A4, B6	(2)	TPCEGSWFFCTL	23
E2		LGCDVIYWSSGWGCKF	31
B3		GSCSLSIVTIPCGV	30
D3		VTCAAFSLFPFCYN	28
E7		CHPGWPCFW	28
D8		GGCNLSVLWCLD	27
F10		NECILVNGDRWCIS	27
E6		FPCWALPSQYLCRP	27
D11		TACNLSVSWCEF	20
G11		TNCGFLYLGCVI	18
E9		FDCGDGSWRYYLDCVR	18
B7		TSCRVLWIMTLCNA	18
B10		EVCNNKLWPCFW	16
A8		GGCGWLWVSCVI	14
A2		CSPAWPCWW	14
F2		LPCAGRSFPCYW	14
C8		DRCLLWVSLCMA	13
E1		CIPMWPCWW	13

^aIdentified clones and observed frequencies in cases where multiples were observed.
^bThe underlined sequences specifically bound to U-CLL mAb068, but not an unrelated mAb, and retained binding activity in 750 mmol/L NaCl.
^cRatio of ELISA signals for wells coated with U-CLL mAb068 over wells coated with HSA negative control. In cases where a sequence was observed more than once, the average is shown. For all others, results for a single replicate are shown. In general, data among independent experiments were in good agreement.

ISSN: 1528-3658