Figure 1
Systemic administration of GTS-21 increases bacterial clearance and decreases acute lung injury in mice exposed to hyperoxia and challenged with PA. C57BL/6 mice were exposed to ≥99% O2 for 48 h and then inoculated with PA (0.1 × 108 CFUs/mouse) and returned to 21% O2 after inoculation. Mice were randomized to receive either GTS-21 (0.04, 0.4 and 4 mg/kg) or saline, administrated by intraperitoneal injection every 8 h starting at 32 h during hyperoxia. BAL and lung tissue were harvested 18 h after inoculation. Viable bacteria in the airways and lungs were quantified by plating serial dilutions of homogenized lung (A) and BAL (B) and are expressed as log CFUs/lung and log CFUs/mL of BAL, respectively. The total protein content in the BAL (C) was measured as a marker of lung injury. Data represent the mean ± SEM from two independent experiments (n = 5 for control, n = 6 for all GTS-21-treated mice). *p < 0.05, compared with mice receiving normal saline.