Figure 2
GTS-21 restores hyperoxia-compromised phagocytic ability of macrophages. RAW 264.7 cells were either exposed to 95% O2 alone (black bar) or 95% O2 in the presence of different concentrations of GTS-21 (gray bars) or remained at 21% O2 (white bar) for 24 h. Cells were then incubated with FITC-labeled latex minibeads for 1 h and stained with phalloidin to visualize the cells. (A) Immunofluorescent images of RAW 264.7 cells (red: actin cytoskeleton; green: minibeads). At least 250 cells per well from three independent experiments were counted, and the number of beads per cell was represented as percent beads phagocytosed in the bar graph (B). Each value represents mean ± SEM of three independent experiments for each group. *p < 0.05 and **p < 0.01 versus 95% O2 alone (black bar). Bar = 10 um.