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Figure 2 | Molecular Medicine

Figure 2

From: Antiangiogenic and Antitumoral Effects Mediated by a Vascular Endothelial Growth Factor Receptor 1 (VEGFR-1)-Targeted DNAzyme

Figure 2

Molecular and cellular assays for DT18. (A) DT18 was assayed for its kinetic activity under a single turnover condition. Control was made with the same arms and an inverted catalytic core. The first order rate constant (kobs) was used to determine the cleavage efficiency. (B) Transfection efficiency of DT18 was measured using FITC-labeled DT18 in HUVECs by FACS. TMP was used as a transfection reagent. (C) Effect of DT18 on VEGFR-1 expression in HUVECs was examined by Western blot. (D) Matrigel tube formation assay was performed to assess the antiangiogenic activity of DT18.

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Molecular Medicine

ISSN: 1528-3658