| UM-UC-6 | UM-UC-9 |
---|
Gated cells | Dac | DMSO | Sts | Dac | DMSO | Sts |
---|
% Viable | 3 | 64.7 | 2.4 | 35.2 | 66.2 | 27.9 |
% Apoptotic | 73 | 22.1 | 56 | 8.8 | 7.9 | 17.7 |
% Necrotic | 24 | 13.2 | 41.6 | 56 | 25.9 | 54.4 |
- UM-UC-6 and UM-UC-9 cells were analyzed by flow cytometry following a single 48-h treatment of 2 µmol/L dacomitinib (Dac) or equivalent concentration of DMSO. Resulting percentage of gated cells is indicated. Dac induced apoptosis in UM-UC-6, but not in UM-UC-9 cells compared with DMSO treatment. The percentage of necrotic cells increased in both cell lines; 0.5 µmol/L staurosporin (Sts) was used as positive control for apoptosis (p < 0.0001).