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Figure 2 | Molecular Medicine

Figure 2

From: Brain Region-Specific Alterations in the Gene Expression of Cytokines, Immune Cell Markers and Cholinergic System Components during Peripheral Endotoxin-Induced Inflammation

Figure 2

Relative Il1b mRNA expression is differentially increased in various brain regions after peripheral LPS administration. Mice were injected with LPS (8 mg/kg, IP) or saline and euthanized 4 h later. Brain regions were isolated and processed for Il1b mRNA expression analysis by qPCR, as described in Materials and Methods. Data are shown as mean ± SEM and fold-change (n = 15 mice per group); ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05. Because of limited RNA levels in the hippocampus, hypothalamus, striatum and thalamus, three tissue samples (from the same region and treatment group) were combined before RNA extraction; therefore, n = 5 was used for statistical analyses in these regions.

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