Figure 5

Gfap mRNA expression is increased in specific brain regions during lethal inflammation. Mice were injected with LPS (8 mg/kg, IP) or saline and euthanized 4 h later. Brain regions were isolated and processed for Gfap mRNA expression analysis by qPCR, as described in Materials and Methods. Data are shown as mean ± SEM (n = 15 mice per group); ***P < 0.0001, **P < 0.0002, *P < 0.05. Because of limited RNA levels in the hippocampus, hypothalamus, striatum and thalamus, three tissue samples (from the same region and treatment group) were combined before RNA extraction; therefore, n = 5 was used for statistical analysis in these regions.