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Figure 7 | Molecular Medicine

Figure 7

From: Brain Region-Specific Alterations in the Gene Expression of Cytokines, Immune Cell Markers and Cholinergic System Components during Peripheral Endotoxin-Induced Inflammation

Figure 7

Lethal inflammation is associated with morphological alterations in microglia indicative of activation. Mice were injected with LPS (8 mg/kg, IP) or saline and euthanized 4 h later. Brain tissues were stained for Iba1 immunohistochemistry, as described in Materials and Methods. Iba1-stained microglia were evaluated in the cortex and hippocampus by assessing three visual fields per brain region, per mouse. Microglia were faintly stained with anti-Iba1 in the cortex and the hippocampus of saline-injected mice, while morphological changes of activated microglia, including cell body enlargement, shortening and thickening of processes, were observed in these brain areas of LPS-injected mice. Representative photomicrographs (40×) of Iba1-stained sections are shown: cortex-saline (A), cortex-LPS (B), hippocampus-saline (C) and hippocampus-LPS (D). Enlarged single cell images are shown on the upper right corners. Scale bar, 50 µm.

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