Table 2 Expression of HMGB1 in DRG and spinal cord.
From: Extracellular High-Mobility Group Box 1 Protein (HMGB1) as a Mediator of Persistent Pain
Pain model | Species | Tissue | Method | HMGB1 expression | Antibody | Reference |
|---|---|---|---|---|---|---|
Nerve injury pain | Â | Â | Â | Â | Â | Â |
SNL | Rat | DRG | IHC | Naive: IR in satellite cells, neurons, Schwann cells | Rabbit, SHINO-test | 56 |
|  |  |  |  | SNL: ↑ in most neurons |  |  |
| Â | Â | Spinal nerve | IHC | Naive and SNL: IR cells | Â | Â |
Nucleus pulposus | Rat | DRG | WB | Total homogenate: NC | Rabbit, Shino-Test | 76 |
TNI | Rat | DRG | IHC | Naive: nuclear IR in satellite cells, neurons | Rabbit, Sigma-Aldrich | 37 |
|  |  |  |  | TNI: ↑ in cytoplasm (in neurons) |  |  |
|  |  |  | WB | Total homogenate: NC, extranuclear fraction: ↑ |  |  |
Diabetes | Mouse | Spinal cord | WB | Total homogenate: ↑ | Rabbit, BD Pharmingen | 80 |
PSNL | Rat | Spinal cord | IHC | Naive: IR in microglia, astrocytes, neurons | Mouse private | 75 |
|  |  |  |  | PSNL: ↑ in neurons |  |  |
|  |  |  | WB | Total homogenate: ↑ |  |  |
CCI | Rat | Spinal cord | WB | Total homogenate (dorsal horn): ↑ | Rabbit, BD Pharmingen | 99 |
CCI | Rat | Spinal cord | WB | Total homogenate: ↑ | Rabbit, Abcam | 100 |
Inflammatory pain | Â | Â | Â | Â | Â | Â |
LPS paw | Rat | DRG | WB | Total homogenate: NC | Rabbit, SHINO-test | 61 |
Arthritis | Mouse | Spinal cord | WB | Total homogenate: NC | Rabbit, Abcam | 65 |
|  |  |  | WB | Extranuclear fraction: ↑ |  |  |
| Â | Â | Â | IHC | Naive: IR in microglia, astrocytes, neurons | Â | Â |
Other pain conditions | Â | Â | Â | Â | Â | Â |
Bone cancer | Rat | Spinal cord | WB | Total homogenate (dorsal horn): ↑ | Rabbit, BD Pharmingen | 79 |