Figure 1

Delayed wound healing in diabetic rats. (A) Two 5.0-mm wounds were created on the dorsal surface in normal and streptozotocin-induced diabetic rats (n = 6), and the progress of wound closure was followed until 12 d postwounding. (B) The wound area in both groups of animals was measured at each time point and the mean values are presented. Each point is the mean ± SEM of six animals. (C) Normal and diabetic wounded skin tissues (d 7 postwounding) were paraffin-embedded and sections (5 µm) were stained with H&E. Presence of inflammatory cells in the wounded diabetic skin is shown by red arrows (magnification 20x). (D) Inflammatory cells in both normal and diabetic wound (d 7 postwounding) were quantified as described in Materials and Methods, and results are presented as the number of inflammatory cells per mm² area. **p < 0.01 and ***p < 0.001 compared with wounds in normal animals on the same day.