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Figure 2 | Molecular Medicine

Figure 2

From: Downregulation of miRNAs during Delayed Wound Healing in Diabetes: Role of Dicer

Figure 2

Downregulation of miRNAs in wounds of diabetic rats on d 7 postwounding. (A) Total RNA was isolated from normal and diabetic rat skin (unwounded) (n = 6, isolated on the same day as collection of wound tissues on d 7 postwounding), 500 ng RNA was reverse-transcribed and quantitative PCR was done on a TaqMan Rodent miRNA card, as described in Materials and Methods. The significantly (p < 0.05) altered miRNAs (in the diabetic rat skin compared with the normal rat skin) along with their fold changes are shown. (B) In the volcano plot of the above RT-PCR array, the green triangles are the downregulated miRNAs and the red squares are the upregulated miRNAs. (C) Total RNA was isolated from wounds of normal and diabetic rats (n = 6) on the seventh day postwounding and reverse-transcribed (500 ng), and quantitative PCR for miRNAs was done as in (A). miRNAs altered in the wounds of diabetic rat skin (compared with normal rat wound on d 7 postwounding) together with their fold changes is shown and the volcano plot for the experiment is given in (D) (downregulated miRNAs are green triangles). miRNAs enclosed within black rectangular boxes (in C) are common to those altered in the diabetic unwounded skin (compared with normal rat unwounded skin, A). All miRNAs in (A) and (C) are significantly altered with p ≤ 0.05 and values are means ± SD. FC, fold change; w.r.t., with respect to.

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