Figure 1

Pretreatment of mice with aloperine attenuates IR-induced renal injury. Sham-operated mice were served as a negative control. (A) Serum creatinine (Cr) levels. (B) Levels for blood urea nitrogen (BUN). Aloperine-pretreated mice manifested significantly lower Cr and BUN as compared with that of mice from the IR group. (C) Results for HE staining of renal sections. The severity of renal injury was assessed as semiquantitative scores based on inflammatory infiltration and tubular necrosis. (D) Results for PAS staining of renal sections. Similarly, the severity of renal injury was assessed as semiquantitative scores based on the integrity of brush border and basement membrane. Cast formation was assessed as mean ± SEM of six mice analyzed for each group. Scale bar = 100 µm. *, P < 0.05; **, P < 0.01; and P < 0.001.