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Figure 6 | Molecular Medicine

Figure 6

From: Aloperine Protects Mice against Ischemia-Reperfusion (IR)-Induced Renal Injury by Regulating PI3K/AKT/mTOR Signaling and AP-1 Activity

Figure 6

Aloperine regulates PI3K/Akt/mTOR signaling and NF-κB transcriptional activity. (A) Results for analysis of total and phosphorylated PI3Kp85 subunit in renal lysates. (B) Total and phosphorylated Akt in renal lysates. (C) Analysis of total and phosphorylated mTOR in renal lysates. (D) Western blot analysis of nuclear NF-κBp65 activity in the kidneys. (E) Analysis of NF-κBp65 activity in RAW264.7 cells that had undergone hypoxic insult in the presence of aloperine. (F) EMSA results for analysis of NF-κB DNA binding activity in RAW264.7 cells that had undergone hypoxic insult in the presence of aloperine. Panels (A–D) present quantitative analysis as mean ± SEM of six mice analyzed for each group. *, P < 0.05; **, P < 0.01; and P < 0.001.

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Molecular Medicine

ISSN: 1528-3658