Figure 7

The impact of aloperine pretreatment on AP-1 activity. (A) Analysis of nuclear AP-1 subunit c-Fos expression in renal lysates. (B) Western blot results for analysis of Bcl-2 in renal lysates. (C) Analysis of nuclear c-Fos levels in HK2 cells that had undergone hypoxic insult in the presence of aloperine along with administration of TanIIA. (D) Western blot analysis of Bcl-2 expression in the above prepared HK2 cells. (E) EMSA results for analysis of AP-1 DNA-binding activity in the above prepared HK2 cells. (F) TanIIA abrogated aloperine-induced IL-10 expression in RAW264.7 cells following hypoxic insult. (G) Western blot analysis for total and phosphorylated Jak2 levels in renal lysates of mice that had undergone IR insult. (H) Total and phosphorylated Stat3 in the kidneys of mice after IR insult. Panels (A, B, G, H) present quantitative analysis as mean ± SEM of six mice analyzed for each group.*, P < 0.05; **, P < 0.01; and P < 0.001.