Galantamine alters cytokine responses in immunized mice. KLH immunized mice were administered galantamine (4 mg/kg) or saline 4 h before death. Splenocytes were extracted, plated at 2 × 105 in 96-well plates and incubated with indicated µg/well KLH. Cytokines were determined on d 7 by Quansys multiplex ELISA. (A) Galantamine did not significantly affect IL-2. (B) IL-4 (p < 0.001) and IL-6 (p < 0.0001) were significantly different in galantamine-treated animals (two-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001, by Bonferroni posttest).