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Figure 1 | Molecular Medicine

Figure 1

From: Transgenic Expression of miR-222 Disrupts Intestinal Epithelial Regeneration by Targeting Multiple Genes Including Frizzled-7

Figure 1

Characterization of miR222-Tg mice. (A) Expression of the miR-222/GFP bicistronic RNA as detected by RT-PCR in the mucosa of stomach, small (S) intestine, colon, cecum, liver, spleen, kidney and thymus of 8-wk-old littermate (a) and miR222-Tg mice (b). Total RNA was isolated and used in RT reactions. RT-PCR products of miR222/GFP were visualized in ethidium bromide-stained agarose gels; low-level amplification of GAPDH served as controls. (B) In situ detection of miR-222/GFP using anti-GFP antibody in the small intestine. Scale bar: 50 µm. (C) Levels of miR-222 in various tissues as measured by Q-PCR analysis. Values are means ± SEM of data from five animals. *P < 0.05 compared with littermates.

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Molecular Medicine

ISSN: 1528-3658