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Figure 5 | Molecular Medicine

Figure 5

From: Transgenic Expression of miR-222 Disrupts Intestinal Epithelial Regeneration by Targeting Multiple Genes Including Frizzled-7

Figure 5

miR-222 directly interacts with the Fzd7 mRNA. (A) Immunoblots of FZD proteins in the small intestinal mucosa. (B) Fzd mRNA levels in the mucosa. Values are the mean ± SEM (n = 5). (C) Schematic representation of Fzd7 mRNA depicting predicted target sites for miR-222 in its coding region (CR). (D) miR-222 and U6 levels in IEC-6 cells transfected with biotinylated miR-222 for 24 h. Values are mean ± SEM (n = 3). *P < 0.05 compared with cells transfected with scramble. (E) Levels of Fzd7, Cdk2, JunD and Myc mRNAs in the materials pulled down by biotin-miR-222.

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Molecular Medicine

ISSN: 1528-3658