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Figure 6 | Molecular Medicine

Figure 6

From: Transgenic Expression of miR-222 Disrupts Intestinal Epithelial Regeneration by Targeting Multiple Genes Including Frizzled-7

Figure 6

miR-222 represses FZD7 translation. (A) miR-222 levels 48 h after transfection with pre-miR-222. Values are mean ± SEM (n = 3). *P < 0.05 compared with scramble. (B) Immunoblots of FZD7 protein (a) and its mRNA as measured by Q-PCR analysis (b) in cells described in (A). (C) Newly synthesized FZD7 protein as measured by L-azidohomoalaine (AHA) kit. Top, representative immunoblots of newly synthesized FZD-7; bottom, quantitative analysis of the immunoblotting signals as measured by densitometry. Values are mean ± SEM of data from three separate experiments. (D) Distributions of Fzd7(top) and Gapdh (bottom) mRNAs in each gradient fraction. Nuclei were pelleted, and the resulting supernatants were fractionated through a 10–50% linear sucrose gradient. The levels of Fzd7 and Gapdh mRNAs were plotted as a percentage of the total Fzd7 or Gapdh mRNA levels in the samples. (E) Levels of reporter activities (n = 6).

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