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Figure 7 | Molecular Medicine

Figure 7

From: Transgenic Expression of miR-222 Disrupts Intestinal Epithelial Regeneration by Targeting Multiple Genes Including Frizzled-7

Figure 7

miR-222 inhibits epithelial repair in IEC-6 cells cocultured with Wnt5a-transfected fibroblasts after wounding. (A) Imaging pictures of epithelial repair: scramble (a) and cells (b) transfected with pre-miR-222 for 48 h. The monolayer was wounded, and plates were photographed immediately or 16 h thereafter. (B) Summarized data in cells described in (A). Values are the mean ± SEM (n = 6). *,+P < 0.05 compared with 0 h repair and 16 h repair of scramble-transfected cells, respectively. (C) Epithelial repair 48 h after silencing FZD7: (a) C-siRNA transfection and (b) siFzd7 transfection. (D) Summarized data from cells described in (C). (E) Changes in Wnt-dependent transcription activity as measured by pMyc-TA-luciferase reporter gene assays 8 h after wounding with or without transfection with pre-miR-222 (left) or siFzd7 (right).

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