Figure 5

Metformin and GSK3β inhibitor SB216763 affect T172-AMPK phosphorylation in lungs of mice subjected to sepsis. Representative Western blots and quantitative data show the extent of pT172-AMPK, pS485-AMPK, pT479-AMPK and AMPK downstream target pS79-ACC in lung homogenates obtained from mice treated with (A,B,C) metformin or (C,D,E) GSK3β inhibitor SB216763 and then subjected to CLP-induced sepsis (means ± SD, n = 3-5, *p < 0.05, NS = not significant). (G) Cross-talk between metformin-stimulated pT172-AMPK and inhibitory IKKβ/GSK3β-induced S485/T479-AMPK phosphorylation modulates AMPK activity in lung of mice subjected to abdominal sepsis.