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Figure 2 | Molecular Medicine

Figure 2

From: Temporary CXCR3 and CCR5 Antagonism Following Vaccination Enhances Memory CD8 T Cell Immune Responses

Figure 2

SDS-PAGE and western blot analysis of purified full-length NP protein expressed in Escherichia coli. (A) SDS-PAGE and Coomassie staining. Lane 1: purified NP protein; lane 2: bacterial whole cell lysate without IPTG induction; lane 3: bacterial whole-cell lysate with IPTG induction. (B) Western blot analysis. Purified NP protein and uninduced bacterial whole-cell lysate were detected with 1:5,000 diluted mouse anti-His tag monoclonal antibodies (Sigma) and 1:30,000 diluted goat-anti mouse Abs-conjugated to alkaline phosphatase (Sigma). Lanes 1, 2, 3: purified NP protein; lane 4: bacterial whole-cell extract without IPTG induction.

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